Part:BBa_C0083:Experience

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User Reviews

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••• iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562017). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in E. coli using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part.

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Review number not understood iGEM Nagahama_2014

BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. We removes LVA tag in BBa_C0083 resulting to new parts BBa_K1342002 And we detected and quantified of aspartic acid synthesized by E. coliJM109/BBa_K1342001 by TLC.

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