Coding
Part:BBa_K1499400:Design
Designed by: Ian Hull Group: iGEM14_StanfordBrownSpelman (2014-10-09)
Chitin-binding wasp protein 1 (PdomMRNAr1.2-03231.1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 143
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence for this gene was predicted through peptide mass fingerprinting of Polistes dominula nest protein samples. We believe it to be a potential candidate for the salivary protein that the wasps use to build and waterproof their nests. We have codon-optimized it for E. coli expression.
Source
Polistes dominula