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Coding
mRFP1
Part:BBa_E1010
Designed by: Drew Endy Group: Antiquity (2004-07-28)
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
Usage and Biology
Robert E. Campbell started with Discosoma RFP (DsRed) and evolved a faster folding, monomeric variant. See paper listed in source. Codon optimized for expression in bacteria (?? DE)
iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), amilGFP BBa_K592010 (yellow) and RFP BBa_E1010 (red).
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/bsubtilis
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/fluorescence
//test
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/fluorescence
//test
abs | |
biology | |
color | Red |
direction | Forward |
emission | 607 |
emit | 607 |
excitation | 584 |
excite | 584 |
kegg | |
lum | |
protein | mRFP1 |
swisspro | |
tag | None |