Generator
Part:BBa_K1073014
Designed by: iGEM Team Braunschweig 2013 Group: iGEM13_Braunschweig (2013-09-16)
Constitutively expressed transcription regulator RhlR + LVA
This device consists of a strong constitutive promoter (BBa_J23100) combined with RBS (BBa_B0032) and the coding region of the transcription regulator RhlR (BBa_C0071). This results in a constitutively high transcription level of RhlR.
Usage and Biology
In combination with N-buturyl homoserine lactone (N-butyryl-HSL) synthesized by RhlI, RhlR is able to activate the inducible promoter of the rhl quorum sensing system of Pseudomonas aeruginosa.
RhlR binds to specific sequences in the promoter region and activates gene expression in the presence of N-butyryl-HSL [Medina et al., 2003].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 302
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 777
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Categories
Parameters
None |