Coding

Part:BBa_J03210:Design

Designed by: James Brown   Group: iGEM2005   (2006-08-08)
Revision as of 16:21, 8 August 2006 by Registry (Talk | contribs)

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malE [Maltose-binding Protein]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 133


Design Notes

The sequence information was acquired from Genbank and the physical DNA shipped from the Larson Lab, Stockholm. PCR was used to produce BioBrick


Source

Swedish Centre for Bioprocess technology, Stockholm Ref: Larson, G.(2004) "Solubility and proteolysis of the Zb-malE and Zb-malE31 proteins during overproduction in Escherichia coli."

Biotechnology & Bioengineering Volume 90, Issue 2, Pages 239-247

References