Coding

Part:BBa_J03210:Design

Designed by: James Brown   Group: iGEM2005   (2006-08-08)


malE [Maltose-binding Protein]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 133


Design Notes

The sequence information was acquired from NCBI and the physical DNA shipped from the Larson Lab, Stockholm. PCR was used to produce BioBrick

Source

Swedish Centre for Bioprocess technology, Stockholm Ref: Larson, G.(2004) "Solubility and proteolysis of the Zb-malE and Zb-malE31 proteins during overproduction in Escherichia coli."

Biotechnology & Bioengineering Volume 90, Issue 2, Pages 239-247

References

Larson, G.(2004) "Solubility and proteolysis of the Zb-malE and Zb-malE31 proteins during overproduction in Escherichia coli."

Biotechnology & Bioengineering Volume 90, Issue 2, Pages 239-247