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Device
Part:BBa_K1065106:Design
Designed by: Caterina Marchioretti Group: iGEM13_UNITN-Trento (2013-08-31)
Wintergreen device with SAM: Plac + PCHBA + araCpBAD+ BSMT1 + Plac + SAM synthetase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3107
Illegal BamHI site found at 3856
Illegal BamHI site found at 4736 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 389
Illegal NgoMIV site found at 793
Illegal NgoMIV site found at 918
Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 1210
Illegal NgoMIV site found at 1678
Illegal AgeI site found at 2942
Illegal AgeI site found at 5316 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3397
Illegal SapI site found at 2924
Design Notes
The part was created subcloning BBa_K1065105 inside BBa_K1065102 following the standard assembly protocol.
Source
BBa_J45320 contains PchBA
BBa_K1065101 contains AraC-pBAD and BSMT1
SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.
References
- Chang-Kui Ding, Chien Yi Wang (2003). The dual effects of methyl salicylate on ripening and expression of ethylene biosynthetic genes in tomato fruit, Plant Science Volume 164, Pages 589–596.
- Yang, S. F. at al.(1965), Ethylene biosynthesis and its regulation in higher plants Plant Physiol, 35, 155-89.
- Lutz R, Bujard H.(1997) Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. Mar 15;25(6):1203-10.
- Lee et al., Mechanism of araC autoregulation and the domains of two overlapping promoters, PC and PBAD, in the L-arabinose regulatory region of Escherichia coli, PNAS, Vol. 78, Num. 2, 752-756.
- Wang et al., (2002) Ethylene biosynthesis and signaling networks; Ecker J. R. Plant Cell , Supplement, S131-S151