Part:BBa_K1065106:Design

Wintergreen device with SAM: Plac + PCHBA + araCpBAD+ BSMT1 + Plac + SAM synthetase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3107
  Illegal BamHI site found at 3856
  Illegal BamHI site found at 4736
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 389
  Illegal NgoMIV site found at 793
  Illegal NgoMIV site found at 918
  Illegal NgoMIV site found at 929
  Illegal NgoMIV site found at 1210
  Illegal NgoMIV site found at 1678
  Illegal AgeI site found at 2942
  Illegal AgeI site found at 5316
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3397
  Illegal SapI site found at 2924
  

Design Notes

The part was created subcloning BBa_K1065105 inside BBa_K1065102 following the standard assembly protocol.

Source

BBa_J45320 contains PchBA

BBa_K1065101 contains AraC-pBAD and BSMT1

SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.

References

Chang-Kui Ding, Chien Yi Wang (2003). The dual effects of methyl salicylate on ripening and expression of ethylene biosynthetic genes in tomato fruit, Plant Science Volume 164, Pages 589–596.

Yang, S. F. at al.(1965), Ethylene biosynthesis and its regulation in higher plants Plant Physiol, 35, 155-89.

Lutz R, Bujard H.(1997) Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. Mar 15;25(6):1203-10.

Lee et al., Mechanism of araC autoregulation and the domains of two overlapping promoters, PC and PBAD, in the L-arabinose regulatory region of Escherichia coli, PNAS, Vol. 78, Num. 2, 752-756.

Wang et al., (2002) Ethylene biosynthesis and signaling networks; Ecker J. R. Plant Cell , Supplement, S131-S151