Device

Part:BBa_K1065106:Design

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-08-31)
Revision as of 22:20, 3 October 2013 by Fabius924 (Talk | contribs) (→‎References)


Wintergreen device with SAM: Plac + PCHBA + araCpBAD+ BSMT1 + Plac + SAM synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3107
    Illegal BamHI site found at 3856
    Illegal BamHI site found at 4736
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3397
    Illegal SapI site found at 2924


Design Notes

The part was created subcloning BBa_K1065105 inside BBa_K1065102 following the standard assembly protocol.

Source

BBa_J45320 contains PchBA

BBa_K1065101 contains AraC-pBAD and BSMT1

SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.

References

1. Chang-Kui Ding, Chien Yi Wang (2003). The dual effects of methyl salicylate on ripening and expression of ethylene biosynthetic genes in tomato fruit, Plant Science Volume 164, Pages 589–596.
2. Yang, S. F. at al.(1965), Ethylene biosynthesis and its regulation in higher plants Plant Physiol, 35, 155-89.



Lutz R, Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10.