Regulatory
Part:BBa_K1067007
Designed by: Kristian Davidsen and Helen Cook Group: iGEM13_DTU-Denmark (2013-09-28)
Tight pBAD with araC No leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as part:BBa_K808000 but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the pBAD SPL from DTU iGEM team 2013.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1182
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1182
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1182
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
[edit]
Categories
Parameters
//cds/transcriptionalregulator/activator
//regulation/positive
//regulation/positive
positive_regulators |