Reporter

Part:BBa_K1073022

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 11:31, 28 September 2013 by JonasZ (Talk | contribs) (→‎Usage and Biology)

Constitutively expressed chromoprotein eforRed

The device consists of a strong promoter (BBa_J23100) combined with a RBS (BBa_B0032) and the encoding region of the chromoprotein eforRed. This results in a constitutively high expression of eforRed.


Usage and Biology

During cultivation, the chromoprotein eforRed is expressed and stored intracellularly. The red color of the protein can be seen by naked eye, thus you can use the chromoprotein as a reporter or selection marker and distinguish cells easily.

Expressed chromoproteins.png EforRed pellet.png

iGEM Team Braunschweig 2013:

Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP (BBa_K1073024), eforRed and aeBlue (BBa_K1073020).

Right: Cell pellet of E. coli XL1 expressing eforRed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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