Part:BBa_K1162009:Experience
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how you used this part and how it worked out.
Applications of BBa_K1162009
Experience This part was cloned behind the lac inducible promoter and rbs system BBa_K208010. (https://parts.igem.org/wiki/index.php?title=Part:BBa_K208010) After which GFP (BBa_K208000 https://parts.igem.org/wiki/index.php?title=Part:BBa_K208000) was place behind the His Tag. Fluorescence was observed after induction on a UV light box demonstrating that the His Tag has been fully translated. To demonstrate that the His Tag was functional a lysed cell fraction was passed through a Nickel Resin and His Tagged GFP was bound to the column.
User Reviews
UNIQ063f9f2290c276bb-partinfo-00000000-QINU UNIQ063f9f2290c276bb-partinfo-00000001-QINU