Tag

Part:BBa_K1162009:Experience

Designed by: Kathleen Miller   Group: iGEM13_Utah_State   (2013-09-15)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1162009

Experience This part was cloned behind the lac inducible promoter and rbs system BBa_K208010. (https://parts.igem.org/wiki/index.php?title=Part:BBa_K208010) After which GFP (BBa_K208000 https://parts.igem.org/wiki/index.php?title=Part:BBa_K208000) was place behind the His Tag. Fluorescence was observed after induction on a UV light box demonstrating that the His Tag has been fully translated. To demonstrate that the His Tag was functional a lysed cell fraction was passed through a Nickel Resin and His Tagged GFP was bound to the column.

User Reviews

UNIQ4dcca9d8755157a7-partinfo-00000000-QINU UNIQ4dcca9d8755157a7-partinfo-00000001-QINU

Utah State iGEM team 2013 demonstrated that this part worked as expected.