Coding

Part:BBa_K1074000:Experience

Designed by: Changlong Zhao   Group: iGEM13_USTC_CHINA   (2013-09-09)
Revision as of 07:03, 27 September 2013 by Nocturne (Talk | contribs) (→‎User Reviews)


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Applications of BBa_K1074000

User Reviews

UNIQ6919e06dec5aa38d-partinfo-00000000-QINU UNIQ6919e06dec5aa38d-partinfo-00000001-QINU

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USTC_China iGEM13 

In our project,we fused TD1 with a varity of antigens(HBsAg(Hepatitis B virus)BBa_K1074002,Ag85b(Mycobacterium tuberculosis)BBa_K1074003,PAD4(Bacillus Anthracis)BBa_K1074004 ),adjuvant LTB (BBa_K1074005)and GFP(BBa_E0040), induced expression in BL21 and Bacillus Subtilis WB800N with IPTG and tested positive by SDS-PAGE and ELISA. Then we validated the  efficient transdermal function of TD1 fused antigen HBsAg by ELISA. Students in Wen lab also  validated the efficient transdermal function of  N-terminal TD1 modified GFP(not BBa_E0040)
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