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Part:BBa_K1073022:Experience

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 20:28, 26 September 2013 by Laura S (Talk | contribs) (→‎Applications of BBa_K1073022)


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Applications of BBa_K1073022

When eforRed is expressed the colonies on agar plates as well as cells liquid culture exhibit a bright pink color. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains when incubated in a mixed culture.

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BBa_K1073022 iGEM Team Braunschweig 2013

The chromoprotein eforRed has a distinct red color. In order to avoid absoption of the chomoproteins during measurement with the photometer, the absorption and emission spectra were measured.

BSAbsorption-eforRed.jpg

Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm.


Absorption maximum for eforRed: 534 nm

Emission maximum for eforRed: 605,4 nm


Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and aeBlue (BBa_K1073020).

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