Coding

Part:BBa_K1031743

Designed by: He Shuaixin   Group: iGEM13_Peking   (2013-09-09)
Revision as of 14:51, 26 September 2013 by Psyche (Talk | contribs)

J23113-PaaX


Construction

In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031743 is composed of three elements, the constitutive Pc promoter J23113[1], coding sequence of PaaX and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Pa/J23113-PaaX. The orange arrowheads represent promoters. RBS are shown in green ovals. The square in dark red stands for terminator B0015.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



[edit]
Categories
//cds/transcriptionalregulator/repressor
//chassis/prokaryote/ecoli
//function/regulation/transcriptional
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
Parameters
negative_regulators