Coding
Part:BBa_K1031743
Designed by: He Shuaixin Group: iGEM13_Peking (2013-09-09)
J23113-PaaX
For detailed information concerning PaaX, please visit 2013 Peking iGEM Biosensor PaaX
Construction
In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031743 is composed of three elements, the constitutive Pc promoter J23113, coding sequence of PaaX and terminator B0015. (Fig 1)
Fig 1 Construction of biosensor circuit Pa/J23113-PaaX. The orange arrowheads represent promoters. RBS are shown in green ovals. The square in dark red stands for terminator B0015.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/transcriptionalregulator/repressor
//chassis/prokaryote/ecoli
//function/regulation/transcriptional
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
//chassis/prokaryote/ecoli
//function/regulation/transcriptional
//regulation/negative
//rnap/prokaryote/ecoli/sigma70
negative_regulators |