Composite

Part:BBa_K1188000

Designed by: Bill Heymann, Phil Jensen   Group: iGEM13_CU-Boulder   (2013-08-14)
Revision as of 17:56, 14 August 2013 by Registry (Talk | contribs)

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AmilCP screening part

This part has a strong constitutive promoter (J23100) the standard RBS (B0034) and AmilCP (K592009). When assembled into a plasmid it gives a strong blue color.

The reason we created this part is the default backones all have essentially the same construct but using RFP instead. With an insert with a different color it made it much easier to debug problems with digests, ligations and transformations. We also used it to try to find a problem with some strange background issues we had.

This was part also use to create a lot of AmilCP that was used in our protein purification experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/color
Parameters
n/aAmilCP screening part