Part:BBa_K902019:Design
S7 micrococcal nuclease
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.
GTTTCTTCGA ATTCGCGGCC GCTTCTAGAT GACAGAGTAT CTCCTAAGCG CGGGCATCTG TATGGCCATT GTTTCGATCT TATTGATCGG CATGGCGATA TCGAACGTAT CTAAGGGCCA GTACGCGAAA CGCTTCTTCT TTTTCGCCAC GAGTTGCCTT GTCTTAACAT TAGTTGTTGT TTCTTCACTG TCGTCCAGTG CGAACGCCTC TCAAACCGAT AACGGTGTAA ATCGCAGCGG GAGCGAAGAT CCTACCGTGT ATTCCGCGAC CTCAACGAAA AAACTTCATA AGGAGCCCGC TACGCTCATC A*A*AGCCATTG ACGGAGACAC TGTAAAGCTG ATGTACAAAG GCCAACCGAT GACCTTTCGC TTGCTGCTGG TCGACACCCC AGAGACGAAA CATCCGAAAA AGGGCGTCGA GAAATACGGC CCGGAAGCAA GTGCGTTTAC CAAGAAGATG GTGGAAAATG CGAACAAAAT TGAAGTCGAA TTTGATAAAG GGCAGCGTAC CGACAAATAT GGTCGTGGAC TGGCCTACAT TTATGCTGAT GGTAAAATGG TGAATGAAGC ACTGGTGCGG CAGGGTCTGG CCAAAGTGGC TTATGTGTAT AAACCGAATA ATACTCACGA ACAGCTGCTG CGTAAATCCG AAGCACAGGC AAAAAAAGAA AAACTGAACA TTTGGAGCGA AGATAACGCG GATAGCGGTC AATAATAATA ATACTAGTAG CGGCCGCTGC AGGAAGAAAC
Source
This part was synthesized from IDT and is native to Staphylococcus aureus