Coding
Part:BBa_K902019:Design
Designed by: Himika Dastidar Group: iGEM12_Calgary (2012-10-02)
S7 micrococcal nuclease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The S7 nuclease was synthesized from IDT however there was a point mutation in the sequence that hindered the activity of the nuclease. Instead of the 'A' there was a 'T'. We did mutagenesis to change the mutation back to its original nucleotide. This brick has the S7 that has undergone mutagenesis and is functional.
Source
This part was synthesized from IDT and is native to Staphylococcus aureus
References
1) NCBI no. 57634611 (S7 nuclease sequence)</br>
2) Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.