Generator
PatgB4HT

Part:BBa_K844016

Designed by: Brian Smith   Group: iGEM12_Utah_State   (2012-10-02)
Revision as of 03:58, 3 October 2012 by Canyoncatalyst (Talk | contribs)

Spider Silk Generator - 4x "B" Silk Construct with His Tag

Protein generator part for Spider Silk. Protein is a 4x fusion of spider silk "B" subunits, which were codon optimized to use a reduced set of tRNA codons (1 to 2 codons per amino acid). Contains a lactose/IPTG inducible , RBS, and a 10x-His tag for purification of protein.

IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below will not reflect (it shows Assembly Standard #10 scars).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/silk
Parameters
None