Other

Part:BBa_K784016

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)
Revision as of 16:39, 21 September 2012 by Hila K R (Talk | contribs)

Phage Lambda fragment 2 - tail proteins

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome.
Fragment 2 contains the phages tail proteins, which together with fragment 1 (BBa_K784015), 3 (Part:BBa_K784017) and 4 (BBa_K784018) create the phage envelope.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 741
    Illegal PstI site found at 1834
    Illegal PstI site found at 1998
    Illegal PstI site found at 3984
    Illegal PstI site found at 4056
    Illegal AgeI site found at 2771
    Illegal AgeI site found at 3156
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3645
    Illegal SapI.rc site found at 923
    Illegal SapI.rc site found at 2591
    Illegal SapI.rc site found at 5507


[edit]
Categories
Parameters
None