Part:BBa_K784016

Phage Lambda fragment 2 - tail proteins

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 2 contains the phages tail proteins, which together with fragment 1 (BBa_K784015), 3 (Part:BBa_K784017) and 4 (BBa_K784018) create the phage envelope.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 741
  Illegal PstI site found at 1834
  Illegal PstI site found at 1998
  Illegal PstI site found at 3984
  Illegal PstI site found at 4056
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 741
  Illegal PstI site found at 1834
  Illegal PstI site found at 1998
  Illegal PstI site found at 3984
  Illegal PstI site found at 4056
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 741
  Illegal PstI site found at 1834
  Illegal PstI site found at 1998
  Illegal PstI site found at 3984
  Illegal PstI site found at 4056
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 741
  Illegal PstI site found at 1834
  Illegal PstI site found at 1998
  Illegal PstI site found at 3984
  Illegal PstI site found at 4056
  Illegal AgeI site found at 2771
  Illegal AgeI site found at 3156
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3645
  Illegal SapI.rc site found at 923
  Illegal SapI.rc site found at 2591
  Illegal SapI.rc site found at 5507