Composite

Part:BBa_K729008

Designed by: Erick Miguel Ramos-Martinez   Group: iGEM12_University_College_London   (2012-07-10)
Revision as of 17:59, 22 September 2012 by Bayke5 (Talk | contribs)

pCstA+RBS+T7RNAP+pT7+RBS+GFP+TT

This starvation sensitive device was used by the [http://2012.igem.org/Team:University_College_London UCL’s 2012 iGem team] to increase the expression of a reporter gene (GFP) under low glucose concentrations. During carbon starvation cAMP binds the cAMP receptor protein, the complex formed activates the expression of the downstream gene (T7 RNA polymerase), which promotes the expression of GFP under the control of the T7 promoter.

This device comprises the ligation of the parts (BBa_K729007) and (BBa_I746909).

-

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2887


[edit]
Categories
Parameters
None