Coding
Bxb1_Xis

Part:BBa_K907001

Designed by: Dong-hui Choe, Soo-in Lee   Group: iGEM12_KAIST_Korea   (2012-09-20)
Revision as of 07:46, 26 September 2012 by Robinald (Talk | contribs)

Mycobacterium Phage Bxb1 gp47, DNA excisionase


KAIST DNA recombination via Bxb1 Int, Xis.png

This part is a protein coding part which encodes DNA excisionase of Mycobacterium phage Bxb1. The Bxb1 excisionase is a DNA recombinase, more precisely a member of serine integrase family. It forms complex with Bxb1 integrase. The complex recognizes specific sequences, called attL and attR, and then catalyzes DNA recombination that is exact reverse reaction done by integrase alone.

<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907002 - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP
BBa_K907005 - Dual Phase Protein Generator(mRFP default). mRFP and GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination
Parameters
None