Composite

Part:BBa_K784046

Designed by: Asaf Blumental   Group: iGEM12_Technion   (2012-09-25)
Revision as of 10:37, 26 September 2012 by Sl.ilya (Talk | contribs)

theophylline riboswitch +T7*(N4) RNAP

This part contains an engineered theophylline riboswitch (RS) (BBa K784005) and an engineered T7*(N4) RNA polymerase (K784044). The riboswitch contains a RBS and it will be exposed and starts translating the T7*(N4) RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(N4) RNAP are adjacent to one another without any restriction site between them.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None