Device

Part:BBa_K786003:Design

Designed by: Leung Wai Tak   Group: iGEM12_Hong_Kong-CUHK   (2012-09-21)
Revision as of 14:00, 21 September 2012 by Registry (Talk | contribs)

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Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
    Illegal NgoMIV site found at 614
    Illegal NgoMIV site found at 638
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 875
    Illegal AgeI site found at 144
    Illegal AgeI site found at 1616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 977


Design Notes

Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRI fusion with HtrII.


Source

From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.

References