Device
Part:BBa_K786003:Design
Designed by: Leung Wai Tak Group: iGEM12_Hong_Kong-CUHK (2012-09-21)
Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 785
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 37
Illegal NgoMIV site found at 614
Illegal NgoMIV site found at 638
Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 875
Illegal AgeI site found at 144
Illegal AgeI site found at 1616 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 977
Design Notes
Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRI fusion with HtrII.
Source
From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.