Composite
Part:BBa_K823052
Designed by: Jara Radeck Group: iGEM12_LMU-Munich (2012-09-10)
lacZ, B. subtilis optimized + RBS + Term
lacZ in Freiburg standard, Bacillus subtilis codon optimized with ribosome binding site and terminator (B0014).
lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).
This BioBrick was used for promoter evaluation in B. subtilis.
Find out more about the design of our prefix with ribosome binding site.
prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC
suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT
We used this lacZ gene to evaluate our promoters PliaG, PliaI, Pveg.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |