Part:BBa_K325909:Experience

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• [http://2012.igem.org/Team:Fudan_Lux 2012iGEM team Fudan_Lux]

Growth curve of K325909 In 2012 iGEM competition we measured growth curve of K325909 in a E.coli strain dH5α. The curve shows that induced by the arabinose, the bacteria growth Check the RAW data of measurement.

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•••• [http://2012.igem.org/Team:Peking/Project/Communication Peking 2012 iGEM team]

We successfully constructed light communication system using this part-luxbrick, and we made supplementary characterization of it. Spectrum We measured the spectrum of luminescence produced by luxbrick. As shown in the spectrum below, the luminescence has a maximum intensity at 485nm. Figure 3. TOP10 cells harboring the luxbrick were cultured in LB medium and induced with L-arabinose at 10-3M. 10 hours after induction, the glowing cells were measured for spectrum using SHIMADZU RF5301PC Spectrofluorophotometer. Time course-video We showed the time course of light emitting process of TOP10 cells transformed with luxbrick in a more visualized way, which is a short video. In the movie, the cells begin to glow 9 hours after induction, and the entire visible glowing process lasts about 10 hours. See the video, please click [http://www.youtube.com/watch?v=fnhe1MKIO9Q&feature=youtu.be here]. (If you can't see the video above, please click [http://v.youku.com/v_show/id_XNDU0NDU0NDcy.html here])

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UNIQ517811f89470af1e-partinfo-00000003-QINU UNIQ517811f89470af1e-partinfo-00000004-QINU