Coding
SYFP2

Part:BBa_K864100

Designed by: Erik Gullberg   Group: iGEM12_Uppsala   (2012-09-24)
Revision as of 22:55, 24 September 2012 by Registry (Talk | contribs)

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Super Yellow Fluorescent Protein 2 (SYFP2)

This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/yfp
//collections/chromoprotein/uppsala
//function/reporter/fluorescence
Parameters
chassisCodon optimized for expression in E coli.
colorYellow
emission527 nm
excitation515 nm