Device

Part:BBa_K886001:Design

Designed by: edgar andres ochoa cruz   Group: iGEM12_USP-UNESP-Brazil   (2012-09-24)
Revision as of 21:36, 24 September 2012 by Registry (Talk | contribs)

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Recombination Device composed by lox71-Cre recombinase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the lox71 submitted by our group, which correct the part BBa_I718017 and BBa_K537020 when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/


Source

We synthesized the lox71 site and then we assembled it with the Cre recombinase part BBa_J61047

References