Device

Part:BBa_K886001:Design

Designed by: edgar andres ochoa cruz   Group: iGEM12_USP-UNESP-Brazil   (2012-09-24)

Recombination Device composed by lox71-Cre recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the lox71 submitted by our group BBa_K886000, which corrected the parts BBa_I718017 and BBa_K537020. Those parts are wrong when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/

Source

We synthesized the lox71 site, then we assembled it upstream the Cre recombinase gene(BBa_J61047).

References

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/

http://2012.igem.org/Team:USP-UNESP-Brazil/Plasmid_Plug_n_Play/Introduction