Regulatory
P-alsT

Part:BBa_K818300

Designed by: Emeraldo Jo   Group: iGEM12_Groningen   (2012-09-19)
Revision as of 11:09, 19 September 2012 by Registry (Talk | contribs)

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Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.

Promoter alsT is a promoter that is repressed by tnrA in the presence of low ammonium by B. subtilis. tnrA is a promoter that responds to ammonium availability in the environment. At low availbility tnrA is activated thus repressing alsT. At high availbility of ammonium (produced by rotten meat), tnrA will be deactivated and alsT will be active.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
  • 1000
    COMPATIBLE WITH RFC[1000]


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