Part:BBa_K818300
Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.
alsT is a promoter in B. subtilis repressed by TnrA. TnrA, in turn, is only active when glutamine synthetase (GS) is actively converting NH4 to glutamine. This in turn is regulated by cell growth, not the amount of NH4/NH3+ present. However, in one article it was observed that GS is also active under conditions of nitrogen limitation. If the medium contained the precise amount of glutamine necessary to support the initial growth phase, then the glutamine would be depleted at the sensing phase. GS would activate to try and create more glutamine. GS would only deactivate when the amount of extracellular NH4/NH3+ reached a level to remove the lack of nitrogen as factor limiting further growth.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 465
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 465
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 465
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 465
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 465
- 1000COMPATIBLE WITH RFC[1000]
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