Composite
psb1C3_int

Part:BBa_K743006:Design

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)
Revision as of 22:34, 20 September 2012 by Registry (Talk | contribs)

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psb1C3_IntK recombination plasmid for Synechocystis PCC6803


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1774
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1774
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1774
    Illegal XhoI site found at 2271
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1774
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

Source

RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance (BBa_P1003) and the double terminator (BBa_B0015) are from the registry of standard biological parts.

References