![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K510044
Designed by: David Caballero Group: iGEM11_UPO-Sevilla (2011-10-21)
pUC18R6KT-miniTn7BB-Gm-bistable_switch
This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the miniTn7BB characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.
For more information about the function of the miniTn7 BioBrick toolkit, visit the page of the pUC18R6KT-miniTn7BB-Gm plasmid (BBa_K510012).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4528
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4528
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal BglII site found at 3769
Illegal BglII site found at 7615 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4528
Illegal XbaI site found at 4543
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 8202
Illegal AgeI site found at 8314 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal BsaI.rc site found at 6171
Illegal SapI site found at 518
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Categories
Parameters
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