Composite

Part:BBa_K510044

Designed by: David Caballero   Group: iGEM11_UPO-Sevilla   (2011-10-21)
Revision as of 18:43, 21 October 2011 by Registry (Talk | contribs)

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pUC18R6KT-miniTn7BB-Gm-bistable_switch

This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the miniTn7BB characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.

For more information about the function of the miniTn7 BioBrick toolkit, visit the page of the pUC18R6KT-miniTn7BB-Gm plasmid (BBa_K510012).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
    Illegal BglII site found at 7615
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 8202
    Illegal AgeI site found at 8314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854
    Illegal BsaI.rc site found at 6171
    Illegal SapI site found at 518


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