Part:BBa_K510044
pUC18R6KT-miniTn7BB-Gm-bistable_switch
This plasmid allows the integration of a transcriptional flip-flop into bacterial chromosomes using the pUC18R6KT-miniTn7BB-Gm (BBa_K510012)characteristics. Single copy may improve the function of regulatory circuits, as bistable systems.
For more information about the function of the [http://2011.igem.org/Team:UPO-Sevilla/Foundational_Advances/MiniTn7/Overview miniTn7 BioBrick toolkit], visit the iGEM team UPO-Sevilla 2011 wiki.
Figure. Fluorescent pellets containing the basic flip-flop device. Pellets of E. coli strains containing the basic flip-flop in the miniTn7BB-Gm transposon in medium copy number (R6K-based vector -part BBa_K510044-, left), high copy number (pUC18-based vector -part BBa_K510043-, center), or not containing the flip-flop device (right). It is quite interesting that the basal state of the basic flip-flop is red in the medium copy number plasmid and green in the high copy number plasmid.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4528
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4528
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal BglII site found at 3769
Illegal BglII site found at 7615 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4528
Illegal XbaI site found at 4543
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 8202
Illegal AgeI site found at 8314 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal BsaI.rc site found at 6171
Illegal SapI site found at 518
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