Composite
Part:BBa_K510042
Designed by: David Caballero, Fernando Govantes Group: iGEM11_UPO-Sevilla (2011-10-19)
pUC18Sfi-miniTn7BB-Gm-Lux
pUC18R6KT-miniTn7BB-Gm-Lux is a vehicle vector for the minitransposon miniTn7BB-Gm-Lux. This transposon is a part of the miniTn7 BioBrick toolkit, a set of plasmids harboring Tn7 derivatives that can be used for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacterial genomes.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4367
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4367
Illegal NheI site found at 5601
Illegal NheI site found at 8567
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4373 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4367
Illegal BglII site found at 3051
Illegal BglII site found at 3322
Illegal BglII site found at 3608
Illegal BglII site found at 7565
Illegal BamHI site found at 5540
Illegal XhoI site found at 8395 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4367
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4367
Illegal XbaI site found at 4382
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 5375 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1681
Illegal BsaI site found at 9954
Illegal BsaI.rc site found at 6963
Illegal SapI site found at 5357
Illegal SapI.rc site found at 2763
Illegal SapI.rc site found at 10279
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Categories
Parameters
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