Composite

Part:BBa_K510042

Designed by: David Caballero, Fernando Govantes   Group: iGEM11_UPO-Sevilla   (2011-10-19)
Revision as of 21:48, 19 October 2011 by Registry (Talk | contribs)

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pUC18Sfi-miniTn7BB-Gm-Lux

pUC18R6KT-miniTn7BB-Gm-Lux is a vehicle vector for the minitransposon miniTn7BB-Gm-Lux. This transposon is a part of the miniTn7 BioBrick toolkit, a set of plasmids harboring Tn7 derivatives that can be used for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacterial genomes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4367
    Illegal NheI site found at 5601
    Illegal NheI site found at 8567
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4367
    Illegal BglII site found at 3051
    Illegal BglII site found at 3322
    Illegal BglII site found at 3608
    Illegal BglII site found at 7565
    Illegal BamHI site found at 5540
    Illegal XhoI site found at 8395
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4367
    Illegal XbaI site found at 4382
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1681
    Illegal BsaI site found at 9954
    Illegal BsaI.rc site found at 6963
    Illegal SapI site found at 5357
    Illegal SapI.rc site found at 2763
    Illegal SapI.rc site found at 10279


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