Device

Part:BBa_K533009

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 14:56, 29 September 2011 by Registry (Talk | contribs)

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Tar receptor weak expression cassette

Tar receptor is now under the drive of lacI promoter, a constitutive weak promoter. In addition, the Shine-Dalgarno sequence is removed to lower translation efficiency. Lower expression level ensures more specific chemotaxis and better engineering of movement control.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1074
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1074
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1074
    Illegal AgeI site found at 1936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2514
    Illegal BsaI site found at 3582
    Illegal SapI.rc site found at 765


[edit]
Categories
Parameters
None