Device

Part:BBa_K533009

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)

Tar receptor weak expression cassette

Tar receptor is now under the drive of lacI promoter, a constitutive weak promoter. In addition, the Shine-Dalgarno sequence is removed to lower translation efficiency. Lower expression level ensures more specific chemotaxis and better engineering of movement control.

Usage and Biology

Mgoulian tar.jpg

pPD10, the empty vector demonstrated no significant chemotaxis while pPD12 vector demonstrated strong chemotaxis. We thus cloned the expression cassette from pPD12 and use it for our chemotaxis experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1074
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1074
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1074
    Illegal AgeI site found at 1936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2514
    Illegal BsaI site found at 3582
    Illegal SapI.rc site found at 765


[edit]
Categories
Parameters
None