Coding
cspB

Part:BBa_K525224

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-12)
Revision as of 13:09, 15 September 2011 by Jaretz (Talk | contribs)

S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7 and RBS

S-layer cspB from Corynebacterium halotolerans with PT7 and RBS

After processing:

Molecular weight: 50.6 kDa

Theoretical pI: 4.25


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 440
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 440
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1191
    Illegal XhoI site found at 647
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 440
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 440
    Illegal NgoMIV site found at 314
    Illegal NgoMIV site found at 1403
    Illegal AgeI site found at 89
    Illegal AgeI site found at 305
    Illegal AgeI site found at 546
    Illegal AgeI site found at 593
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 995
    Illegal BsaI.rc site found at 302
    Illegal BsaI.rc site found at 680
    Illegal BsaI.rc site found at 1082


[edit]
Categories
//chassis/prokaryote/ecoli
//proteindomain/internal
//rnap/bacteriophage/t7
Parameters
None