Deleted
Sample Discontinued
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Plasmid_Backbone
B. S. Vect

Part:BBa_K606003:Design

Designed by: Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-07-25)
Revision as of 15:17, 25 July 2011 by Registry (Talk | contribs)

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Multi-host replicative plasmid, for B. Subtilis and E. Coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
    Illegal SpeI site found at 5918
    Illegal PstI site found at 5904
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 17
    Illegal SpeI site found at 5918
    Illegal PstI site found at 5904
    Illegal NotI site found at 9
    Illegal NotI site found at 5909
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 17
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
    Illegal SpeI site found at 5918
    Illegal PstI site found at 5904
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
    Illegal SpeI site found at 5918
    Illegal PstI site found at 5904
    Illegal NgoMIV site found at 3573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2560
    Illegal BsaI site found at 4550
    Illegal SapI.rc site found at 5632


Design Notes

The EcoRI site has to be eliminated and the Biobricks prefixes and suffixes were recovered


Source

The plasmid was obtained from Harald Putzer laboratory at the IBPC [http://www.ibpc.fr/UPR9073/equipe_HaraldJackie/AccueilJAP_HPGB.htm] . This is the fusion of the commercial plasmids pBC16-1 and pTZ19R via the site EcoRI. This site has been eliminated by the 2011 iGEM Paris Team by quick change mutagenesis, and the multicloning site was transformed into the biobrick standards.

References