Part:BBa_K606003:Design

Multi-host replicative plasmid, for B. Subtilis and E. Coli

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 17
  Illegal XbaI site found at 2
  Illegal SpeI site found at 5918
  Illegal PstI site found at 5904
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 17
  Illegal SpeI site found at 5918
  Illegal PstI site found at 5904
  Illegal NotI site found at 9
  Illegal NotI site found at 5909
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 17
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 17
  Illegal XbaI site found at 2
  Illegal SpeI site found at 5918
  Illegal PstI site found at 5904
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 17
  Illegal XbaI site found at 2
  Illegal SpeI site found at 5918
  Illegal PstI site found at 5904
  Illegal NgoMIV site found at 3573
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 2560
  Illegal BsaI site found at 4550
  Illegal SapI.rc site found at 5632
  

Design Notes

The EcoRI site has to be eliminated and the Biobricks prefixes and suffixes were recovered

Source

The plasmid was obtained from Harald Putzer laboratory at the IBPC [http://www.ibpc.fr/UPR9073/equipe_HaraldJackie/AccueilJAP_HPGB.htm] . This is the fusion of the commercial plasmids pBC16-1 and pTZ19R via the site EcoRI. This site has been eliminated by the 2011 iGEM Paris Team by menaged restriction, completion and ligation, and the multicloning site was transformed into the biobrick standards.