Generator

Part:BBa_K325909:Mutants

Designed by: Theo Sanderson and Will Handley   Group: iGEM10_Cambridge   (2010-10-23)
Revision as of 20:59, 27 October 2010 by PM (Talk | contribs) (→‎Data)

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Input: L-Arabinose
Output: Light

pBad/araC
I0500		PoPS to Light
 Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        H-NS mutants        Add Data       


Description
It has been shown (see References) that the expression of the Vibrio fischeri lux operon when cloned into E. coli was repressed. This repression was linked to the nucleoid protein H-NS. To investigate this effect we cloned the operon into mutant E.coli cells in which the expression of teh H-NS protein had been modified.


Data

Figure 1 - Peak light output from BBa_K325909 cloned into H-NS mutant JM 230 H-NS -205::tn10. The data points and the error bar are the mean and standard deviation obtained by 3 tim repeats.
Figure 2 - Evolution of light output from BBa_K325909 cloned into H-NS mutant JM 230 H-NS -205::tn10 with time at different Arabinose concentrations. The data points and the error bar are the mean and standard deviation obtained by 3 time repeats. Measurements were taken every 30 minutes.
Figure 3 - Figure 3 - Peak light output from BBa_K325909 cloned into H-NS mutant [http://www.ecoliwiki.net/colipedia/index.php/BW25113 BW25113 DELTA H-NS::kan]. The data points and the error bar are the mean and standard deviation obtained by 3 tim repeats.
Figure 4 - Evolution of light output from BBa_K325909 cloned into H-NS mutant [http://www.ecoliwiki.net/colipedia/index.php/BW25113 BW25113 DELTA H-NS::kan] with time at different Arabinose concentrations. The data points and the error bar are the mean and standard deviation obtained by 3 time repeats. Measurements were taken every 30 minutes.
Data Notes Date Uploaded
Media:BBa_K325909Mutants.xls Raw data from experiment 21/10/2010

Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3


References
[http://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291099-1271%28199807/08%2913:4%3C185::AID-BIO486%3E3.0.CO;2-U/abstract [1]:] S. Ulitzur, (1998) H-NS controls the transcription of three promoters of Vibrio fischeri lux cloned in Escherichia coli,Journal of Bioluminescence and Chemiluminescence, 13(4), 185-188.

[http://www.nature.com/nature/journal/v444/n7117/full/nature05283.html [2]:] R.T. Dame et al., (2006) Bacterial chromatin organization by H-NS protein unravelled using dual DNA manipulation,Nature, 444, 387-390.