Part:BBa_K389016

VirA/G reporter device mRFP

This BioBrick contains a complete VirA/G receptor system with an mRFP (BBa_E1010) under the control of a vir promoter as reporter gene.

Input: acetosyringone

Output: expression of mRFP

Usage and Biology

This BioBrick is for testing, characterizing and measuring the VirA/G signaling system. This system contains an unmutated virA, so it is possible to measure how the natural VirA/G system works and reacts. It is also possible to determine the basal transcription of the vir promoter and its activity after inducing the system with acetosyringone.

Important parameters

Experiment	Characteristic	Value
Transfer function	Maximum induction level	2.6 fold
	Maximum induction level reached	150 µM acetosyringone
	Hill coefficient	1.67
	Switch Point	26.5 µM acetosyringone
Doubling time / h	without plasmid	1.98
	carrying K389016	2.57
	carrying K389016 with 150 µM acetosyringone	2.77
	carrying K389016 with 1000 µM acetosyringone	3.01
Conformation analysis	ratio ccc monomer / %	91.2
	ratio ccc dimer / %	3.2
	ratio oc forms / %	5.6
Inducers	Induction by	Acetosyringone
	No induction by	Capsaicin
		Dopamine
		Homovanillic acid
		3-Methoxytyramine

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 647
  Illegal NheI site found at 2581
  Illegal NheI site found at 2604
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1632
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 4260
  Illegal AgeI site found at 4372
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1768