Part:BBa_K332011

Link title Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis.

Bacillus thuringiensis, which is a Gram-positive bacteria. It can produce insecticidal crystal proteins(ICP) which are proteolytically processed by gut proteases into the activated δ-endotoxins. The toxins activated by gut proteases bind to specific binding sites on the brush border membranes of insect midgut epithelial cells. The conformational change in the toxin molecules triggers the insertion of their pore-forming domain into the membrane. Finally, colloid-osmotic swelling and lysis of the cell result in the death of the larvae.

Cry11Aa protein is one of the crystal protein coded in Bacillus thuringiensis subsp. Israelensis and it is highly toxic to certain dipteran larvae, such as Aedes, and Anopheles larvae. The length of Cry11Aa sequence is about 1.9Kb.

In our project, the cry weapon system produce crystal protein, targetting the wrigglers, larvae of mosquitoes. It is controlled by the tetR-repressible promoter PtetR(BBa_R0040)[1], which in turn is regulated by a temperature control system(BBa_K332031)[2]. When E.coli is released into environment (<37°C), tetR begins to degrade, resulting in the promoter PtetR(BBa_R0011)[3] expressing downstream genes, cry11Aa(BBa_K332011)[4] and Green Fluorescent Protein(GFP)(BBa_E0040)[5].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]