Device
pBAD-AB-DT

Part:BBa_K323135:Design

Designed by: Tina Lebar, Tjaa Stoicki   Group: iGEM10_Slovenia   (2010-10-20)
Revision as of 18:16, 23 October 2010 by TinaL (Talk | contribs) (→‎Design Notes)

VioA and VioB enzymes fused with zinc fingers under pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
    Illegal NheI site found at 2568
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 6132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5657
    Illegal AgeI site found at 979
    Illegal AgeI site found at 5853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Part BBa_K323134 was ligated into a vector containing part BBa_K323085 as a back insert. The resulting fragment was then ligated into the BsaI cloning site of part BBa_K323110.

Source

a

References