Coding
Tse2
Part:BBa_K314200:Design
Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger Group: iGEM10_Washington (2010-10-18)
Toxin Tse2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 196
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 378 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
mention something about promoter needs to have no leaky expression for succesful cloning
Source
pseudomonas aeruginosa
References
Cell Host Microbe.�2010 Jan 21;7(1):25-37