Tag

Part:BBa_J70628

Designed by: Joseph Lynch   Group: Knight Lab   (2010-08-11)
Revision as of 20:42, 11 August 2010 by Jolynch (Talk | contribs)

RFC12 Sce VMA Intein Internal Domain This is a RFC12 compatible Intein self-splicing protein. It is capable of self cleavage induced by overnight incubation at 4°C with 50 mM DDT.

Usage and Biology

Intein domains are commonly fused with CBD (BBa_J70627) and then used to cleave off the CBD tag after purification, yielding very high levels of the target protein. Note that the target protein's N terminal or C terminal residue can affect cleavage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 486
    Illegal BamHI site found at 1339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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