Protein_Domain

Part:BBa_J70611:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-07-20)
Revision as of 20:30, 20 July 2010 by Jolynch (Talk | contribs) (→‎Design Notes)

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RFC12 RFP Internal Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 552
    Illegal AgeI site found at 664
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR amplified off of BBa_J04450 with primers: 

GTTTCTT C GAATTC GCGGCCGC T ACTAGT GCTTCCTCCGAAGACGTTATCAAAGAG, RFP-F

GTTTCTT C CTGCAG CGGCCGC T GCTAGC AGCACCGGTGGAGTGACGAC, RFP-R

Source

BBa_E1010

References